BGI HPV

Introduction

Human papilloma virus (HPV) infection is associated with nearly all of cervical cancer cases (99.7%) worldwide[1] . Identification of HPV is an important tool for screening and prevention of carcinoma of the cervix. The BGI HPV Test combines self-sampling technology and genotyping assay to detect 14 most important, “high-risk” types of HPV, including HPV -16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 and 2 “low-risk” types of the virus, HPV -6, 11. In many parts of the world, HPV testing has replaced Pap smear test as the primary screening test for cervical cancer.

  • Gene Panel

14 “high-risk” types of HPV

HPV -16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68

2 “low-risk” types of HPV

HPV -6, 11

  • Sample Requirements
Sample typeStorage mediaQuantity & QualityStorage & Shipment
Cervical exfoliated cellsPE tube≥ 2.5mL, with visible turbidity.Stored and shipped at 2-8°C, reaching BGI lab within 7 days.
CardSample region turns to be white form purple.Stored and shipped at room temperature, reaching BGI lab within 7 days.
DNA (extracted from exfoliated cells)DNA extraction and purification protocol, and composition of storing buffer (such as TE or ddH2O) are required. The storing buffer cannot contain SDS.

Concentration: 25ng/µL,

Volume: ≥ 20 µL OD260/280: 1.8-2.0, no degradation.

Stored and shipped at low temperature, and reaching BGI lab within 7 days with dry ice or icebags. Use enough dry ice to prevent it from complete sublimation before arrival.